Results Specific compounds affected nerve cell axon growth. Cell staining: evaluation of phenotypic changes, three mixed dyes for live cell staining, Calcein AM (1 μM) for vitality staining, MitoTracker Orange (0. After 24 hours of compound cryotubes treatment on the plate, continue to culture under this condition for 3 days, as shown in the figure. It can exhibit the same phenotype and function of primary hepatocytes. After reducing variable factors and other primary cell limitations, the automatic imaging system can Help to obtain and analyze the effect of compounds on hepatocellular toxicity. Obtaining multiple parameters can better describe different cell types and compound effects.
A compact, fully automated microplate image analysis system can be used for multi-parameter experiments, such as the use of induced multifunctional stem cells (iPSC) to differentiate into cardiomyocytes, nerve cells, and liver cells. The PC12 neuroblastoma cell line was used as a model cell for these experiments. This experiment comprehensively demonstrated that ImageXpress Pico automatic cell imaging system and CellReporterXpress software can be applied in cytology-based experiments.
Neuronal cell axon growth and development characteristics are evaluated by their degree of growth (total length or average length per cell), number of nerve axons (total number of axons), degree of branch extension (total number of branches and each Average branch number of cells). Hepatocyte phenotype change iPSC induces human hepatocytes to be a very valuable cell model. The analysis software uses a new logic operation method to identify the target object, which simplifies the analysis process and can provide multi-parameter detection results.2 μM)
for mitochondrial membrane potential dyes, Hoechst (2 μM) for nuclear dyes, all dyes The molecules are all from Life Technologies. Multiple detection methods were used to quantify the complexity of its neuronal cells. Stain with anti-TuJ-1 antibody. Therefore, through this A process to develop new formulations has become the most promising new method of drug therapy. . Similarly, we show some examples of using label-free cell analysis to evaluate cytotoxicity and cell proliferation.
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