If this carbapenem-mimicking compound, CVB-1, is recognized by a carbapenemase, for example, in an bacteria extract, CVB-1 is cleaved and undergoes spontaneous degradation.. Thus, using this fluorescence-based assay system, it would be possible to find out in very short time whether carbapenem-resistant bacteria (such as certain Enterobacteriaceae and Klebsiella pneumoniae strains) are indeed present during an infection.C… Now, Hexin Xie at East China University of Science and Technology and his team have set up a strategy to identify those pathogens that carry the carbapenemases. Xie and his colleagues said: “CVB-1 [. The scientists have performed several tests to prove that their CVB-1 assay is specific, that the detection limit is low, and that it can indeed be used in live systems..They introduce their approach in the journal Angewandte Chemie. As this destroys the electronic interaction of the attached dye with the carbapenem compound, the dye turns into a green fluorescent molecule, which means, China Solar LED tube lamp if it is irradiated with light of a certain wavelength, it emits intense green light. However, even carbapenem-resistant pathogenic strains have emerged over the last decades.Carbapenems are a class of beta-lactam antibiotics similar to cephalosporins and penicillins. To find out whether a pathogen contains carbapenem-cleaving enzymes, the carbapenemases, Chinese scientists have developed a simple and fast assay based on a fluorescent probe and optical detection.], and the addition of [the carbapenemase] triggers the turn-on of the fluorescent signal upon excitation [.Washington D..But there is growing evidence of even carbapenem resistance, and some pathogens were found to produce carbapenem-cleaving enzymes, the carbapenemases. Although some bacterial strains have found powerful strategies to resist beta-lactam antibiotics by producing a class of cleaving enzymes, the beta-lactamases, most beta-lactamases cannot affect the carbapenems..More specific treatment strategies could be designed and an overuse of noneffective drugs could be avoided. Thus, the assay in principle works as follows: If there is an active carbapenemase present, for example, in a bacteria extract, a couple of minutes later the sample glows green upon excitation.: A team of researchers has come up with a fluorogenic probe that can detect the activity of multidrug-resistant pathogens in an assay system.] is essentially non-fluorescent [.] with over 200-fold enhancement ratio.This fast and simple fluorescence-based assay is certainly a remarkable approach in the ongoing and urgent fight against the fast spread of antibiotic resistance.”This technique allows the detection of antibiotic resistance activity by fluorescence.Carbapenems are among the “antibiotics of last resort” and can fight infections for which other drugs have long lost their effectiveness..The researchers developed a molecule that has the same structure as the carbapenems but has a fluorogenic dye attached.Therefore, these substances, which are called “antibiotics of last resort”, are the drug of choice for several diseases such as urinary-tract and abdominal infections as well as hospital-acquired pneumonia, if they are caused by multidrug-resistant bacteria
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