iNGENET - La comunidad de la ingeniería mexicana

The image analysis system is the key point.3 Probe and extinction trials This step can be regarded as an ‘advanced’ step. If a new experiment is not carried out, one batch of inspection part is sufficient. Let the experimental mouse learn and remember the position cryo tube with internal cap of the platform based on the […]

The experimental operation should be performed in a sterile area in the center of the operating table, and do not operate in a non-sterile area at the edge. When dissecting mice, be careful not to damage the spleen and its surrounding organs, especially the intestines, to prevent contamination of the spleen. 8. Hands or relatively […]

In general, small centrifuges place the entire motor on a shock-resistant elastic support, such as rubber pads, etc. ④Improving the centrifuge’s ability to resist imbalances not only improves the robustness of the centrifuge, but also does not require strict balance during the operation of the rotor, and even visual balance can be used, which is […]

This fume hood should be made of wood, plastic or light metal for easy movement. 5. The water supply of the water supply and drainage laboratory follows the principle of convenient access to the nearest place, and a hot water system is installed in the necessary places such as the washing area. The contents involved […]

The monoclonal antibody has the best binding effect when the antigen can maintain its natural conformation. Direct detection is not disturbed by non-specific binding of secondary antibodies, and the operation is simpler when performing multi-color detection. Most cells only need to remove the medium and then add a fixative. However, blocking and control were added […]

Results Specific compounds affected nerve cell axon growth. Cell staining: evaluation of phenotypic changes, three mixed dyes for live cell staining, Calcein AM (1 μM) for vitality staining, MitoTracker Orange (0. After 24 hours of compound cryotubes treatment on the plate, continue to culture under this condition for 3 days, as shown in the figure. It […]

It is recommended to combine at 4 ° C overnight. 10) The antibody concentration is too high or the washing is not enough. It is recommended to reduce the antibody concentration and increase the number and time of washing. 11) The chemical coloring substrate is too much. It is recommended to add an appropriate amount […]