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  • filtertubetipae ha escrito una nueva entrada en el blog: There are many steps for paraffin sectioning   hace 1 semana, 3 días · Ver

    The monoclonal antibody has the best binding effect when the antigen can maintain its natural conformation. Direct detection is not disturbed by non-specific binding of secondary antibodies, and the operation is simpler when performing multi-color detection. Most cells only need to remove the medium and then add a fixative. However, blocking and control were added […]

  • filtertubetipae ha escrito una nueva entrada en el blog: The PC12 neuroblastoma cell line was used as a model cell   hace 3 semanas, 3 días · Ver

    Results Specific compounds affected nerve cell axon growth. Cell staining: evaluation of phenotypic changes, three mixed dyes for live cell staining, Calcein AM (1 μM) for vitality staining, MitoTracker Orange (0. After 24 hours of compound cryotubes treatment on the plate, continue to culture under this condition for 3 days, as shown in the figure. It […]

  • filtertubetipae ha escrito una nueva entrada en el blog: The temperature is too high during electrophoresis   hace 4 semanas · Ver

    It is recommended to combine at 4 ° C overnight. 10) The antibody concentration is too high or the washing is not enough. It is recommended to reduce the antibody concentration and increase the number and time of washing. 11) The chemical coloring substrate is too much. It is recommended to add an appropriate amount […]

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